《植物生理学报》 2015, 51(4): 573-577

大麦籽粒生育酚和生育三烯酚的合成与分布特性

张玉红^1,2, 陈建澍², 曾章慧², 柴玉琼², 柏彬², 韩凝², 边红武², 朱睦元^2,*

¹西藏农牧科学院, 拉萨850000; ²浙江大学生命科学学院, 杭州310058

通信作者：朱睦元；E-mail: myzhu@zju.edu.cn；Tel: 0571-88208623

摘　要：

大麦生育酚(tocopherols, T)和生育三烯酚(tocotrienols, T3)是重要的抗氧化功效成分, 对人体健康具有潜在的应用价值。本研究分析了大麦籽粒发育过程中生育酚和生育三烯酚及其异构体成分的含量和分布特性。结果表明: 在大麦籽粒发育过程中, 籽粒总生育酚和总生育三烯酚含量随籽粒发育进程不断升高, 抽穗后发育第30~35天合成积累速率最快, 占总含量50%左右; 颖壳中的生育酚和生育三烯酚及其异构体含量显著低于去颖壳后籽粒中的含量; 在籽粒发育不同阶段异构体α-T和α-T3含量较高且变化大, 其他6种异构体(β-T、γ-T、δ-T、β-T3、γ-T3和δ-T3)含量低且基本恒定。说明大麦籽粒生育酚和生育三烯酚及其异构体成分的积累速率和分布存在显著差异。

关键词：大麦; 功效成分; 生育酚; 生育三烯酚; 异构体

收稿：2015-02-08   修定：2015-03-08

资助：国家现代农业大麦青稞体系(CARS-05)、浙江省科技项目(2012C12902-2)和西藏自治区科技项目(2010-5)。

Characteristics of Synthesis and Distribution of Tocopherols and Tocotrienols in Barley Grains

ZHANG Yu-Hong^1,2, CHEN Jian-Shu², ZENG Zhang-Hui², CHAI Yu-Qiong², BAI Bing², HAN Ning², BIAN Hong-Wu², ZHU Mu-Yuan^2,*

¹Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China; ²College of Life Sciences, Zhejiang University, Hangzhou 310058, China

Corresponding author: ZHU Mu-Yuan; E-mail: myzhu@zju.edu.cn; Tel: 0571-88208623

Abstract:

Tocopherols (T) and tocotrienols (T3) are important functional compounds in barley with antioxidant activity and potential value for human health. In this study, we analyzed the contents and isomers’ compositions of tocopherols and tocotrienols in barley grains. The results showed that the total content of tocopherols and tocotrienols was gradually increased during grain development in barley and their synthesis and accumulation rates arrived at the highest value at 30–35 day after heading and consequently accounted for 50% of the total. The levels of tocopherols and tocotrienols in grain husks were significantly lower than that in grains with removed husk. Furthermore, at different stages during grain development, α-T and α-T3 displayed a high level and kinetic changes relative to six other isomers including β-T, γ-T, δ-T, β-T3, γ-T3 and δ-T3. These data suggested that accumulation rate of tocopherols, tocotrienols and their isomers are largely distinct during barley grain development.

Key words: barley; functional compounds; tocopherols; tocotrienols; isomers